Composite

Part:BBa_K4388007:Design

Designed by: Tomas Lopes   Group: iGEM22_KCL_UK   (2022-10-09)


Transcriptional Unit 3 in Synthetic Operon for Pterostilbene Production


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For successful ligation in the correct order and orientation, the three basic parts (BBa_K4388011, BBa_K4388003, and BBa_K4388012) were synthesised (IDT) with appropriate JUMP prefixes and suffixes, generating appropriate complementary 4-bp overhangs upon BsaI digestion.


Source

This part was created via JUMP type IIS assembly of basic parts BBa_K4388011, BBa_K4388003, and BBa_K4388012 ligated together into pJUMP29-1C.

References